miR?28?3p inhibits prostate cancer cell proliferation, migration and invasion, and promotes apoptosis by targeting ARF6

Previous studies have reported that the expression levels of microRNA (miR)?28?3p are downregulated in prostate cancer (PCa) compared with those adjacent normal tissues. However, to best our knowledge, function and underlying mechanisms miR?28?3p PCa not been reported. The present study aimed explore role its mechanism development PCa. In study, ADP?ribosylation factor 6 (ARF6) were analyzed using reverse transcription?quantitative PCR (RT?qPCR). Cell proliferation, colony formation, apoptosis, migration invasion determined Counting Kit?8, forming, flow cytometry Transwell assays, respectively. association between ARF6 was investigated a dual luciferase reporter assay. ARF6, Rac1, Erk1/2 phosphorylated (p)?Erk1/2 protein western blotting. results revealed downregulated, whereas upregulated cell lines (LNCaP, 22Rv?1, PC?3 DU145) line RWPE?1. overexpression promoted inhibited invasion. knockdown exerted opposite results. RT?qPCR blotting indicated target gene miR?28?3p. Finally, rescue experiments demonstrated attenuated effects mimic by upregulating Rac1 p?Erk1/2 cells. conclusion, these findings may inhibit biological ehaviors cells targeting therefore represent novel therapeutic candidate for